Part:BBa_K115033:Experience

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Applications of BBa_K115033

These constructs were used to test our RNA thermometers, by measuring luciferase activity after growing at certain temperatures. The fold increase of luciferase activity between cultures of cells grown at 20ºC and 37ºC is displayed in figure 1.

In this figure, 12 (the leftmost) is construct K115012, the reference strain, which has no thermosensitive RNA, but just B0032 as ribosome binding site. This is to compare for normal temperature induced behaviour in cells. The other constructs from 29-36 are K115029 to K115036.

This part was not sent in as there was no luciferase expression at all. Probably during 3A assembly somewhere a functional unit was lost.

For more documentation, visit our wiki [http://2008.igem.org/Team:TUDelft (link)].

Figure 1. Fold increase of luminescence per ug of total protein of each construct in respect to luminescence measured at 20ºC. Numbers in the legend represent the last two numbers of the construct name, e.g. 12 = BBa_K115012. Four samples were measured in duplo for every data point. Error bars represent two times SEM.

User Reviews

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